Abstract

Sesamin enhances the DHA content of tissue lipids in salmonid fish. We have examined whether this effect also occurs in common carp (Cyprinus carpio). Two-year-old common carps were fed a diet containing 5.8 g sesamin kg-1 feed for 69 days. No significant differences were found in the fish weight, specific growth rate, food conversion ratio and lipid content in white muscle. The experimental diet with sesamin increased the percentage of 18:3 n-3 and decreased 18:4 n-3, 22:5 n-3, 22:6 n-3 as well as the desaturation index reflecting the ratio of n-3 long-chain polyunsaturated fatty acids/18:3 n-3 in the triacylglycerol fraction. Sesamin increased the total cytochrome P450 content in hepatopancreatic microsomes as well as 7-ethoxyresorufin O-deethylase (EROD) activity. However, a detailed microarray analysis using 26K gene probes failed to establish any significant pattern of transcriptional response, including lipid biosynthetic genes. Together, these results indicate that under conditions of our study, sesamin was ineffective in the common carp as a means of achieving the changes in the tissue lipid composition as were seen in salmonids.

Keywords

cytochrome P450; EROD; linolenic acid; microarray; sesamin; transcriptomics

Published in

Aquaculture Research
2010, Volume: 41, number: 11, pages: e851-e861

SLU Authors

• Mraz, Jan

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    
  • University of South Bohemia in České Budějovice

• Schlechtriem, Christian

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    
  • Fraunhofer Institute for Molecular Biology and Applied Ecology (IME)

• Zlabek, Vladimir

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    
  • University of South Bohemia in České Budějovice

• Pickova, Jana

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    
  • University of South Bohemia in České Budějovice

UKÄ Subject classification

Food Science
Fish and Aquacultural Science


Publication identifier

DOI: https://doi.org/10.1111/j.1365-2109.2010.02609.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/32020