Comparison of UPLC and HPLC for Analysis of Dietary Folates

Abstract

Ultra performance liquid chromatography (UPLC) using small sub-2 mu m particles and high performance liquid chromatography (HPLC) were compared for separation and determination of the most common dietary folates; 5-methyltetrahydrofolate, tetrahydrofolate, 5-formyltetrahydrofolate, 10-formylfolic acid and folic acid. Two UPLC columns-Acquity BEH C(18) and Acquity HSS T3, and two HPLC columns with similar surface chemistry-Xbridge C(18) and Atlantis d18 were tested. When using UPLC, the signal-to-noise ratio could be improved by a factor of 2-50 for different folate derivatives and the run time could be reduced fourfold without sacrificing separation efficiency. The applicability of UPLC to real food samples was demonstrated

Keywords

Ultra performance liquid chromatography; Ultra fast separation; Sub-2 mu m particles; Folate food analysis LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; HIGH-TEMPERATURE; HIGH-PRESSURE; DERIVATIVES; STABILITY; BERRIES; FINLAND; ASSAY; ACID

Published in

Chromatographia
2011, Volume: 73, number: 3-4, pages: 219-225

SLU Authors

• Jastrebova, Jelena

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Strandler, Hanna Sara

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Patring, Johan

  • Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
    
  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Food Science


Publication identifier

DOI: https://doi.org/10.1007/s10337-010-1896-z

Permanent link to this page (URI)

https://res.slu.se/id/publ/33622