Eriksson, Staffan
- Department of Molecular Biosciences, Swedish University of Agricultural Sciences
Abstract
Background: Thymidine kinase 1 (TK1) is a cytoplasmic enzyme, produced only in the S-phase of proliferating cells, that has potential as a tumor marker. Specific determination of TK1 in serum is difficult, in part because of differences in the physical properties of serum TK1 compared with cytoplasmic TK1. Methods: The first step in the new assay was phosphorylation of 3'-azido-2',3'-deoxythymidine (AZT) to AZT 5'-monophosphate (AZTMP) by TK1 present in patient material. The AZTMP formed was measured in a competitive immunoassay with specific anti-AZTMP antibodies and AZTMP-labeled peroxidase. Results were compared with those of a TK radioenzyme assay (REA) for 78 samples from patients suffering from hematologic diseases. Results: The detection limit was 78 muIU/L, and within-run CVs
Published in
Clinical Chemistry
2004, volume: 50, number: 9, pages: 1597-1606
Publisher: AMER ASSOC CLINICAL CHEMISTRY
SLU Authors
Publication identifier
- DOI: https://doi.org/10.1373/clinchem.2003.030379
Permanent link to this page (URI)
https://res.slu.se/id/publ/3560