Lundh, Åse
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
Abstract
Two surface plasmon resonance (SPR)-based biosensor assays for detection of beta-lactam antibiotics in milk are reported. The assays are based on the enzymatic activity of a carboxypeptidase converting a 3-peptide into a 2-peptide, a reaction that is inhibited in the presence of beta-lactams. Antibodies were used to measure either the amount of formed enzymatic product or the amount of remaining enzymatic substrate. Both assays detected different beta-lactams at or below European maximum residue limits (MRLs), and the detection limit for penicillin G was 1.2 mug/kg and 1.5 mug/kg for the 2- and 3-peptide assays, respectively. The precision (CV) was < 5%, both within and between assays at the penicillin G MRL (4,mug/kg). The biosensor results obtained upon analysis of incurred milk samples were compared with results obtained by liquid chromatography (HPLC), and the method agreements were, in general, good.
Keywords
beta-lactams; milk; beta-lactam receptor protein; SPR biosensor
Published in
Journal of Agricultural and Food Chemistry
2004, volume: 52, number: 10, pages: 2791-2796
Publisher: AMER CHEMICAL SOC
SLU Authors
Gustavsson, Eva
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
- Department of Molecular Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Food Science
Publication identifier
- DOI: https://doi.org/10.1021/jf0344284
Permanent link to this page (URI)
https://res.slu.se/id/publ/3650