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Research article2004Peer reviewed

Nuclear reassortment between vegetative mycelia in natural populations of the basidiomycete Heterobasidion annosum

Johannesson H, Stenlid J

Abstract

Somatic incompatibility is not an absolute block to nuclear exchange between incompatible mycelia of the basidiomycete Heterobasidion annosum in vitro. Under laboratory conditions new heterokaryotic genotypes can be isolated from the gap between incompatible heterokaryons, and nuclear migration between pairs of heterokaryons grown in Petri dishes has been observed. In this study, we test the hypothesis of nuclear transfer and reassortment between heterokaryotic mycelia in natural populations of H. annosum. We developed six microsatellite markers to genotype nuclei populating 21 somatically incompatible mycelia of H. annosum isolated from a single stump of Picea abies, and found that the detected heterokaryons share nuclei; 10 of the nuclear haplotypes were found in more than one mycelium. In one isolate, four nuclear types were found in the same mycelium. These findings indicate that new heterokaryons can be formed as a result of nuclear reassortment between incompatible heterokaryotic mycelia in nature. (C) 2004 Elsevier Inc. All rights reserved

Keywords

Nuclear mosaic; Nuclear reassortment; Somatic incompatibility; Microsatellites; Root rot; Natural chimera; Fungi; Allorecognition; Individual; Operational selective unit

Published in

Fungal Genetics and Biology
2004, Volume: 41, number: 5, pages: 563-570
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

    UKÄ Subject classification

    Environmental Sciences related to Agriculture and Land-use

    Publication identifier

    DOI: https://doi.org/10.1016/j.fgb.2004.01.002

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/4106