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Research article2013Peer reviewed

In vitro inhibition of 7-ethoxyresorufin-O-deethylase (EROD) and p-nitrophenol hydroxylase (PNPH) activities by sesamin in hepatic microsomes from two fish species

Wagner, L; Zlabek, V; Trattner, S; Zamaratskaia, G

Abstract

In the present study, we investigated the effect of sesamin on CYP1A (7-ethoxyresorufin-O-deethylase, EROD) and CYP2E1-like activities (p-nitrophenol hydroxylase, PNPH) in hepatic microsomes obtained from Atlantic salmon (Salmo salar) and common carp (Cyprinus carpio). Addition of sesamin to the incubations in a concentration range from 1 to 200 mu M decreased the activities of EROD and PNPH in a concentration dependent manner. It is likely that the inhibition of EROD was mechanism-based as demonstrated by the decrease in the IC50 value from 5.9 to 3.2 mu M for A. salmon and from 7.9 to 3.0 mu M for common carp when 5 min pre-incubation step was included. Similarly, PNPH activity was inhibited by sesamin with a decrease in the IC50 values from 61.7 to 15.2 mu M for A. salmon and from 194.3 to 20.7 mu M for common carp. Thus, our results indicated that sesamin can act as a mechanism-based inhibitor of EROD and PNPH activity with similar degree of inhibition in both fish species. More importantly, the inhibition of CYP1A, in addition to being mechanism-based, was competitive with K-i value of 5.3 mu M.

Keywords

Atlantic salmon; Common carp; CYP1A; CYP2E1-like protein; Mechanism-based inhibition

Published in

Molecular Biology Reports
2013, volume: 40, number: 1, pages: 457-462

SLU Authors

UKÄ Subject classification

Ecology
Fish and Aquacultural Science

Publication identifier

  • DOI: https://doi.org/10.1007/s11033-012-2080-3

Permanent link to this page (URI)

https://res.slu.se/id/publ/41326