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Research article2012Peer reviewed

An Optimized Method for Analysis of Phenolic Compounds in Buds, Leaves, and Fruits of Black Currant (Ribes nigrum L.)

Vagiri, Michael; Ekholm, Anders; Andersson, Staffan; Johansson, Eva; Rumpunen, Kimmo


Although the fruits are the economic driver for the black currant industry, the buds and leaves are excellent sources of beneficial phenolic compounds that may contribute to the future value of the crop. In this study, extraction of phenolic compounds for different parts of the black currant plant was optimized, and an efficient method for their separation by HPLC was developed. This allowed the simultaneous quantification of a range of hydroxycinnamic acids, flavan-3-ols, flavonols, and anthocyanins by DAD following their identification by HPLC-ESI-MSn. A total of 23 compounds were detected in the buds, 22 of which were found in fruit and leaves. To the best of our knowledge, this is the first report of flavonol glycosides of quercetin, myricetin, isorhamnetin, and kaempferol along with hydroxycinnamic acids such as neo-chlorogenic acid and chlorogenic acid in the buds. Additionally, we provide the first evidence of kaempferol-3-O-rutinoside in black currant leaves. This approach offers avenues for superior combined compositional identification and cultivar selection targeted at the generation of polyphenol-rich products derived from the whole crop and not just the fruit.


anthocyanins; ascorbic acid; bioactive compounds; extraction; flavonol; functional food; HPLC; polyphenols

Published in

Journal of Agricultural and Food Chemistry
2012, Volume: 60, number: 42, pages: 10501-10510