Mast cells express tyrosine hydroxylase and store dopamine in a serglycin-dependent manner

Abstract

Here we show that mast cells contain dopamine and that mast cell activation causes dopamine depletion, indicating its presence within secretory granules. Dopamine storage increased during mast cell maturation from bone marrow precursors, and was dependent on the presence of serglycin. Moreover, the expression of tyrosine hydroxylase, the key enzyme in dopamine biosynthesis, was induced during mast cell maturation; histidine decarboxylase and tryptophan hydroxylase 1 were also induced. Mast cell activation caused a robust induction of histidine decarboxylase, but no stimulation of tyrosine hydroxylase or tryptophan hydroxylase 1 expression. The present study points toward a possible role of dopamine in mast cell function.

Keywords

dopamine; histidine decarboxylase; mast cells; serglycin; tryptophan hydroxylase; tyrosine hydroxylase

Published in

Biological Chemistry
2012, volume: 393, number: 1-2, pages: 107-112
Publisher: WALTER DE GRUYTER & CO

SLU Authors

• Pejler, Gunnar

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Rönnberg, Elin

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Immunology
Biochemistry and Molecular Biology

Publication identifier

• DOI: https://doi.org/10.1515/BC-2011-220

Permanent link to this page (URI)

https://res.slu.se/id/publ/42323