Skip to main content
Research article - Peer-reviewed, 2012

Comparison of three fluorescent CYP3A substrates in two vertebrate models: pig and Atlantic salmon

Zlabek, Vladimir; Zamaratskaia, Galia


We investigated in vitro inhibitory effects of ketoconazole (KTZ) on cytochrome P450 activity in microsomes from pigs and Atlantic salmon. The following enzymatic reactions were studied: 7-benzyloxyresorufin and 7-ethoxyresorufin O-dealkylation (BROD and EROD, respectively), 7-benzyloxy-4-trifluoromethylcoumarin O-debenzylation (BFCOD) and 7-benzyloxyquinoline O-debenzylation (BQOD). KTZ was a potent non-competitive inhibitor of BROD and BQOD in the microsomes from pigs, whereas in the microsomes from Atlantic salmon, these reactions were competitively inhibited by KTZ. BFCOD activity was inhibited by KTZ in a non-competitive manner in both species. KTZ non-competitively inhibited EROD in Atlantic salmon, but not in porcine microsomes. The activity of BROD and BQOD was higher in male than that in female pigs, but the activity of BFCOD showed no sex-related differences.


ketoconazole; 7-ethoxyresorufin; 7-benzyloxyresorufin; 7-benzyloxy-4-trifluoromethylcoumarin; 7-benzyloxyquinoline

Published in

2012, volume: 6, number: 4, pages: 633-640

Authors' information

Zlabek, Vladimir
Swedish University of Agricultural Sciences, Department of Food Science
Swedish University of Agricultural Sciences, Department of Food Science

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication Identifiers


URI (permanent link to this page)