Abstract

We investigated in vitro inhibitory effects of ketoconazole (KTZ) on cytochrome P450 activity in microsomes from pigs and Atlantic salmon. The following enzymatic reactions were studied: 7-benzyloxyresorufin and 7-ethoxyresorufin O-dealkylation (BROD and EROD, respectively), 7-benzyloxy-4-trifluoromethylcoumarin O-debenzylation (BFCOD) and 7-benzyloxyquinoline O-debenzylation (BQOD). KTZ was a potent non-competitive inhibitor of BROD and BQOD in the microsomes from pigs, whereas in the microsomes from Atlantic salmon, these reactions were competitively inhibited by KTZ. BFCOD activity was inhibited by KTZ in a non-competitive manner in both species. KTZ non-competitively inhibited EROD in Atlantic salmon, but not in porcine microsomes. The activity of BROD and BQOD was higher in male than that in female pigs, but the activity of BFCOD showed no sex-related differences.

Keywords

ketoconazole; 7-ethoxyresorufin; 7-benzyloxyresorufin; 7-benzyloxy-4-trifluoromethylcoumarin; 7-benzyloxyquinoline

Published in

Animal
2012, Volume: 6, number: 4, pages: 633-640
Publisher: CAMBRIDGE UNIV PRESS

SLU Authors

• Zlabek, Vladimir

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Zamaratskaia, Galia

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1017/S1751731111002096

Permanent link to this page (URI)

https://res.slu.se/id/publ/42472