Abstract

The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof.

Published in

Application number: US13/441,118
Patent number: US8278416B1
Application published date: 2012-10-02
Publication date: 2012-10-02

SLU Authors

• Johansson, Jan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Hjälm, Göran

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Stark, Margareta

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Rising, Anna

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Grip, Stefan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Engström, Wilhelm

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Hedhammar, My

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)


More information

Assignee: Spiber Technologies AB, Uppsala (SE)


Permanent link to this page (URI)

https://res.slu.se/id/publ/42809