Moritz, Thomas
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Research article1995Peer reviewed
Comparison between high-resolution selected ion monitoring, selected reaction monitoring, and four-sector tandem mass spectrometry in quantitative analysis of gibberellins in milligram amounts of plant tissue
Moritz, Thomas; Olsen, Jorunn
Abstract
Gibberellins (GAs) were quantified in semipurified plant extracts by combined gas chromatography/mass spectrometry(GC/MS). High-resolution selected ion monitoring (HR-SIM), selected reaction monitoring (SRM), and four sector tandem mass spectrometry (MS/MS) were compared. The best selectivity was found with four-sector MS/MS analysis, but the sensitivity was too low for analysis in milligram amounts of plant tissue. HR-SIM and SRM had similarly low limits of detection, except for the analyses of GA(29), but SRM provided better selectivity. The variations of sensitivity and selectivity for the different MS methods are discussed.
Published in
Analytical Chemistry
1995, Volume: 67, number: 10, pages: 1711-1716
Publisher: AMER CHEMICAL SOC
SLU Authors
UKÄ Subject classification
Analytical Chemistry
Publication identifier
DOI: https://doi.org/10.1021/ac00106a010
Permanent link to this page (URI)
https://res.slu.se/id/publ/42908