Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates

Sammanfattning

Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU. (C) 2011 Elsevier B.V. All rights reserved.

Nyckelord

Active bacteria; BrdU incorporation; Replicating bacteria; Soil bacteria; Thymidine analog

Publicerad i

Journal of Microbiological Methods
2011, Volym: 86, nummer: 3, sidor: 376-378 Utgivare: ELSEVIER SCIENCE BV

SLU författare

• Hellman, Maria

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Hallin, Sara

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

UKÄ forskningsämne

Jordbruksvetenskap
Fisk- och akvakulturforskning
Miljö- och naturvårdsvetenskap


Publikationens identifierare

DOI: https://doi.org/10.1016/j.mimet.2011.05.020

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/46481