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Forskningsartikel - Refereegranskat, 2011

Survey of bromodeoxyuridine uptake among environmental bacteria and variation in uptake rates in a taxonomically diverse set of bacterial isolates

Hellman, Maria; Berg, Jeanette; Brandt, Kristian K.; Hallin, Sara


Incorporation of 5-Bromo-2'-Deoxyuridine (BrdU) into DNA can be used to target replicating bacteria in the environment, but differential uptake capacity is a potential bias. Among 23 bacterial isolates commonly found in soils, most took up BrdU, but at up to 10-fold different cell-specific rates. Combined with results from an in silico analysis of 1000 BrdU-labeled 16S rRNA gene sequences, our results demonstrate a BrdU uptake bias with no apparent relationship between taxa affiliation and ability to incorporate BrdU. (C) 2011 Elsevier B.V. All rights reserved.


Active bacteria; BrdU incorporation; Replicating bacteria; Soil bacteria; Thymidine analog

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Journal of Microbiological Methods
2011, Volym: 86, nummer: 3, sidor: 376-378