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Research article2004Peer reviewed

Expression, purification, crystallization and preliminary X-ray diffraction studies of the cmcl component of Streptomyces clavuligerus 7 alpha-cephem-methoxylase

Lester DR, Oster LM, Svenda M, Andersson I

Abstract

Cephamycins are broad-spectrum beta-lactam antibiotics that show resistance to certain forms of beta-lactamases. They differ from cephalosporins by the presence of a methoxyl group at the C-7alpha position. The gene products of cmcI and cmcJ are believed to control 7alpha-methoxylation of cephalosporins through successive steps of hydroxylation and methylation. Here, the expression, purification, crystallization and initial data-collection statistics of the 236-amino-acid protein product of cmcI from Streptomyces clavuligerus is reported. The crystals belong to space group P2(1), with unit-cell parameters a = 93.6, b = 182.6, c = 103.2 Angstrom, beta = 91.05degrees. Diffraction data were collected to 2.5 Angstrom

Published in

Acta Crystallographica Section D: Biological Crystallography
2004, volume: 60, pages: 1618-1621
Publisher: BLACKWELL MUNKSGAARD

SLU Authors

  • Lester, Diane

    • Department of Molecular Biology, Swedish University of Agricultural Sciences

  • Andersson, Inger

    • Department of Molecular Biology, Swedish University of Agricultural Sciences

  • Öster, Linda

    • Department of Molecular Biology, Swedish University of Agricultural Sciences

Publication identifier

  • DOI: https://doi.org/10.1107/S090744490401666X

Permanent link to this page (URI)

https://res.slu.se/id/publ/4781