Research article - Peer-reviewed, 2004
Expression, purification, crystallization and preliminary X-ray diffraction studies of the cmcl component of Streptomyces clavuligerus 7 alpha-cephem-methoxylase
Lester DR, Oster LM, Svenda M, Andersson IAbstract
Cephamycins are broad-spectrum beta-lactam antibiotics that show resistance to certain forms of beta-lactamases. They differ from cephalosporins by the presence of a methoxyl group at the C-7alpha position. The gene products of cmcI and cmcJ are believed to control 7alpha-methoxylation of cephalosporins through successive steps of hydroxylation and methylation. Here, the expression, purification, crystallization and initial data-collection statistics of the 236-amino-acid protein product of cmcI from Streptomyces clavuligerus is reported. The crystals belong to space group P2(1), with unit-cell parameters a = 93.6, b = 182.6, c = 103.2 Angstrom, beta = 91.05degrees. Diffraction data were collected to 2.5 AngstromPublished in
Acta Crystallographica Section D: Biological Crystallography2004, volume: 60, pages: 1618-1621
Publisher: BLACKWELL MUNKSGAARD
Authors' information
Lester, Diane
Swedish University of Agricultural Sciences, Department of Molecular Biology
Andersson, Inger
Swedish University of Agricultural Sciences, Department of Molecular Biology
Öster, Linda
Swedish University of Agricultural Sciences, Department of Molecular Biology
Svenda, Martin
Swedish University of Agricultural Sciences, Department of Molecular Biology
Publication Identifiers
DOI: https://doi.org/10.1107/S090744490401666X
URI (permanent link to this page)
https://res.slu.se/id/publ/4781