High-Resolution H-1 Magic Angle Spinning NMR Spectroscopy of Intact Arctic Char (Salvelinus Alpinus) Muscle. Quantitative Analysis of n-3 Fatty Acids, EPA and DHA

Abstract

The lipid and small metabolite profiles from intact muscles of Arctic char were investigated using H-1 high-resolution magic angle spinning (H-1 HR-MAS) NMR spectroscopy. Not only the total n-3 fatty acid content but also the eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents of the muscle were obtained from the H-1 HR-MAS NMR spectra without pretreatment of the tissue or lipophilic extraction. A number of small metabolites could also be observed, where creatine/phosphocreatine, anserine and taurine were the most abundant. Thus, the use of H-1 HR-MAS NMR led to simplified analysis techniques that can give direct information on the nutritional value of the fish.

Keywords

Arctic char; Salvelinus alpinus; fatty acids; metabolites; muscle; tissue; H-1 HR-MAS NMR

Published in

Journal of Agricultural and Food Chemistry
2010, Volume: 58, number: 20, pages: 10799-10803
Publisher: AMER CHEMICAL SOC

SLU Authors

• Nestor, Gustav

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Bankefors, Johan

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Schlechtriem, Christian

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Brännäs, Eva

  • Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences
    

• Pickova, Jana

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Sandström, Corine

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Food Science


Publication identifier

DOI: https://doi.org/10.1021/jf103338j

Permanent link to this page (URI)

https://res.slu.se/id/publ/47835