Studies on the interactions between glycosylated beta(3)-peptides and the lectin Vicia villosa by saturation transfer difference NMR spectroscopy

Abstract

Saturation transfer difference (STD) NMR spectroscopy was used to study the interaction of the lectin Vicia villosa (VVLB4) with alpha-D-GalNAc glycosylated beta(3)-peptides. The data were compared to those obtained with the monosaccharides D-Gal, D-GalNAc, and D-Glc as well as with those obtained with the Tn antigen alpha-glycopeptide (D-GalNAc-alpha-O-Ser/Thr), molecule naturally recognized by V. villosa. Evidence that the lectin also recognizes glycosylated beta(3)-peptides and has close contact with both the sugar and amino acid moieties was obtained. (C) 2009 Elsevier Ltd. All rights reserved.

Keywords

STD NMR; Glycosylated beta(3)-peptides; Tn antigen; Lectin Vicia villosa (isolectin B-4)

Published in

Carbohydrate Research
2009, volume: 344, number: 18, pages: 2577-2580
Publisher: ELSEVIER SCI LTD

SLU Authors

• Sandström, Corine

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Organic Chemistry
Biochemistry
Molecular Biology

Publication identifier

• DOI: https://doi.org/10.1016/j.carres.2009.06.040

Permanent link to this page (URI)

https://res.slu.se/id/publ/48887