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Forskningsartikel - Refereegranskat, 2013

The glyoxylate cycle is involved in pleotropic phenotypes, antagonism and induction of plant defence responses in the fungal biocontrol agent Trichoderma atroviride

Dubey, Mukesh; Broberg, Anders; Sooriyaarachchi, Sanjeewani; Ubhayasekera, Wimal; Jensen, Dan Funck; Karlsson, Magnus

Sammanfattning

Isocitrate lyase (ICL), a signature enzyme of the glyoxylate cycle, is required for metabolism of non-fermentable carbon compounds like acetate or ethanol, and virulence in bacteria and fungi. In the present study, we investigate the role of the glyoxylate cycle in the fungal biocontrol agent Trichoderma atroviride by generating id deletion and complementation mutants. Phenotypic analyses of the deletion mutant Delta icl suggest that ICL is required for normal growth, conidial pigmentation and germination, and abiotic stress tolerance. The Delta icl strain display reduced antagonism towards Botrytis cinerea in plate confrontation assays. Secretion and sandwich assays further show that secreted factors are partly responsible for the reduced antagonism. Furthermore, in vitro root colonization assays shows that the Delta icl strain retains the ability to internally colonize Arabidopsis thaliana roots. However, the Delta icl strain has a reduced ability to induce systemic defence in A. thaliana leaves that results in reduced protection against B. cinerea. These data shows that ICL and the glyoxylate cycle are important for biocontrol traits in T. atroviride, including direct antagonism and induction of defence responses in plants. (C) 2013 Elsevier Inc. All rights reserved.

Nyckelord

Antagonism; Biocontrol; Glyoxylate cycle; Isocitrate lyase; Trichoderma atroviride

Publicerad i

Fungal Genetics and Biology
2013, Volym: 58-59, sidor: 33-41
Utgivare: ACADEMIC PRESS INC ELSEVIER SCIENCE

      SLU författare

    • Dubey, Mukesh

      • Institutionen för skoglig mykologi och patologi, Sveriges lantbruksuniversitet

            • Jensen, Dan Funck

              • Institutionen för skoglig mykologi och patologi, Sveriges lantbruksuniversitet

              • Karlsson, Magnus

                • Institutionen för skoglig mykologi och patologi, Sveriges lantbruksuniversitet

              UKÄ forskningsämne

              Microbiology
              Genetics

              Publikationens identifierare

              DOI: https://doi.org/10.1016/j.fgb.2013.06.008

              Permanent länk till denna sida (URI)

              https://res.slu.se/id/publ/50734