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Research article - Peer-reviewed, 2013

The unique structure of complete lipopolysaccharide isolated from semi-rough Plesiomonas shigelloides O37 ( strain CNCTC 39/89) containing (2S)-O-(4-oxopentanoic acid)-alpha-D-Glcp (alpha-D-Lenose)

Kaszowska, Marta; Jachymek, Wojciech; Lukasiewicz, Jolanta; Niedziela, Tomasz; Kenne, Lennart; Lugowski, Czeslaw


in which alpha-D-Lenp is (2S)-O-(4-oxopentanoic acid)-alpha-D-Glcp residue which has not been found in nature. The absolute configuration of oxopentanoic acid moiety in alpha-D-Lenose residue was determined by NOESY experiment combined with molecular modeling (MM2 force field). The decasaccharide core is substituted at C-4 of the beta-D-Glcp residue with a single pentasaccharide unit. Lipid A is built of a beta-D-GlcpN4P-(1 -> 6)-alpha-D-GlcpN1P disaccharide asymmetrically substituted with fatty acids. It was concluded that the core oligosaccharide and the lipid A are identical with those in P. shigelloides CNCTC 113/ 92 Niedziela et al. (2002)(9) and Lukasiewicz et al. (2006).(10) (C) 2013 Published by Elsevier Ltd.


Semi-rough lipopolysaccharide; Oxopentanoic acid; NMR; MALDI-TOF MS; MM2 force field; Plesiomonas shigelloides

Published in

Carbohydrate Research
2013, Volume: 378, pages: 98-107

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    Structural Biology

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