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Research article - Peer-reviewed, 2013

Increased concentrations of C-reactive protein but not high-mobility group box 1 in dogs with naturally occurring sepsis

Karlsson, Iulia; Wernersson, Sara; Ambrosen, Aime; Kindahl, Hans ; Södersten, Fredrik; Wang, Liya; Hagman, Ragnvi

Abstract

Sepsis is difficult to diagnose and remains a common mortality cause worldwide in both humans and animals. The uterine infection pyometra causes sepsis in more than half of affected dogs and therefore allows the natural physiological development of sepsis to be studied. To find a sepsis-specific biochemical marker that could be combined with conventional clinical criteria for a more robust and quick diagnosis of sepsis, we measured systemic concentrations of high-mobility group box 1 (HMGB1) in 23 healthy control dogs and in 27 dogs with pyometra, 74% of which had sepsis. We also measured concentrations of the major acute phase protein C-reactive protein (CRP) and an indicator for endotoxaemia, prostaglandin F-2 alpha metabolite (PGM) to assess the relative contribution of HMGB1 to the detection of systemic inflammation and endotoxaemia. We found that HMGB1 concentrations, in line with concentrations of CRP and PGM, were significantly increased in dogs with pyometra, and that concentrations of CRP, but not HMGB1, were significantly higher in dogs with sepsis compared to dogs without sepsis. Although serum HMGB1 did not differ between dogs with or without sepsis and was not correlated with either CRP or PGM concentrations, HMGB1 was correlated with the total white blood cell counts, suggesting an independent regulation and involvement in inflammation. (C) 2013 Elsevier B.V. All rights reserved.

Keywords

Sepsis; SIRS; Biomarkers; Diagnostics; Cytokines; Canine/dog; Pyometra; Bacterial uterine infection; Inflammation; HMGB1; CRP; PGM

Published in

Veterinary Immunology and Immunopathology
2013, volume: 156, number: 1-2, pages: 64-72
Publisher: ELSEVIER SCIENCE BV

Authors' information

Karlsson, Iulia
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Ambrosen, Aime
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Kindahl, Hans (Kindahl, Hans )
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Swedish University of Agricultural Sciences, Department of Clinical Sciences

UKÄ Subject classification

Immunology
Infectious Medicine
Other Veterinary Science
Biochemistry and Molecular Biology

Publication Identifiers

DOI: https://doi.org/10.1016/j.vetimm.2013.09.011

URI (permanent link to this page)

https://res.slu.se/id/publ/53374