Sammanfattning

The saponin mixture QH-B from the tree Quillaja saponaria var. Molina was fractionated by RP-HPLC in several steps. The fractions were analyzed by solid-phase extraction NMR (SPE-NMR), a technique combining the workup by solid-phase extraction with on-line coupling to an NMR flow probe. Together with MALDI-TOF mass spectrometry and comparison with chemical shifts of similar saponins, the structures of both major and minor components in QH-B could be obtained. The procedure described is a simple method to determine the structure of components in a complex mixture. The two major fractions of the mixture were found to contain at least 28 saponins, differing in the carbohydrate substructures. Eight of these have not previously been determined. The 28 saponins formed 14 equilibrium pairs by the migration of an O-acyl group between two adjacent positions on a fucosyl residue

Publicerad i

Analytical Chemistry
2003, Volym: 75, nummer: 2, sidor: 268-274
Utgivare: AMER CHEMICAL SOC

SLU författare

• Nyberg, Nils

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

• Kenne, Lennart

  • Institutionen för molekylära vetenskaper, Sveriges lantbruksuniversitet
    

Publikationens identifierare

DOI: https://doi.org/10.1021/ac025725v

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/550