Expression of four canine leukocyte adhesion factors in fresh and stored whole blood samples evaluated using a no-lyse, no-wash method

Abstract

Expression of four leukocyte adhesion factors on canine leukocytes was studied by flow cytometry using a no-lyse, no-wash method. The effect of fixation and storage for up to 14 days in 1% paraformaldehyde on labelled samples and within assay variation was evaluated. Monoclonal antibodies directed to monocyte marker CD14, and to adhesion molecules CD11 a, CD18,CD32 and CD49d were used. Cell surface marker, cell population, time, and the interactions between time and cell marker significantly affected expression of cell adhesion factors. For CD18, there was a significant difference in mean fluorescence intensity (MFI) between fresh and stored samples (P

Keywords

Flow cytometry; Leukocyte; Cell surface; Activation; Inflammation

Published in

Veterinary Immunology and Immunopathology
2011, volume: 139, number: 139, pages: 271-276
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Ström Holst, Bodil

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Hagberg Gustavsson, Malin

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Lilliehöök, Inger

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Johannisson, Anders

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Clinical Science

Publication identifier

• DOI: https://doi.org/10.1016/j.vetimm.2010.09.011

Permanent link to this page (URI)

https://res.slu.se/id/publ/57197