Wallgren, Margareta
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
- Quality Genetics
Abstract
Aliquots of ejaculates were spiked with PCV2 and processed by a double processing technique, consisting of Single Layer Centrifugation on Androcoll (TM)-P followed by a "swim-up" procedure. Samples were collected from the resulting fractions during the selection process and analyzed for the presence of infectious PCV2. Virus titres were determined by performing a 50% tissue culture infective dose assay (TCID(50)) by end point dilution and with the use of an indirect peroxidise monolayer assay technique. With an initial infectious virus titre of 3.25-3.82 (TCID(50))/50 mu L the two-step sperm selection method eliminated 2.92 +/- 0.23 logs of infectious PCV2, corresponding to more than 99% reduction. Sperm quality was not affected by the selection procedure. (C) 2011 Elsevier B.V. All rights reserved.
Keywords
Porcine circovirus; Semen; Viral removal; Colloid centrifugation; Swim-up
Published in
Animal Reproduction Science
2011, volume: 126, number: 1-2, pages: 108-114
Publisher: ELSEVIER SCIENCE BV
SLU Authors
Morrell, Jane
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
- Department of Clinical Sciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Animal and Dairy Science
Veterinary Science
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1016/j.anireprosci.2011.04.014
Permanent link to this page (URI)
https://res.slu.se/id/publ/58358