Abstract

phi,beta-Limit dextrins of domains and clusters of sweetpotato amylopectin were subjected to extensive hydrolysis by Bacillus amyloliquefaciens alpha-amylase to release building blocks and reveal the internal structures of clusters. The composition of building blocks was analyzed by size-fractionation, gel permeation chromatography, and high performance anion exchange chromatography. Different domains and clusters had structurally similar building blocks with around three chains per building block and internal chain length around 2.9. Singly branched and doubly branched building blocks were the largest and second largest groups in the clusters. Type A clusters had more large building blocks and contained 5-6 blocks per cluster with an inter-block chain length (IB-CL) of 7.0, whereas type B clusters had less large building blocks and contained 3-4 blocks per cluster with IB-CL 7.9. Models on how the building blocks could be organized into type A and type B clusters are discussed. (C) 2011 Elsevier Ltd. All rights reserved.

Keywords

Building block; Cluster; Bacillus amyloliquefaciens alpha-amylase; Sweetpotato amylopectin structure; C-Type starch

Published in

Carbohydrate Research
2011, Volume: 346, number: 18, pages: 2913-2925
Publisher: ELSEVIER SCI LTD

SLU Authors

• Åman, Per

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Bertoft, Eric

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Organic Chemistry
Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1016/j.carres.2011.10.009

Permanent link to this page (URI)

https://res.slu.se/id/publ/59023