Xia, Hongyan
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Abstract
This study describes a novel blocking microsphere-based immunoassay for highly sensitive and specific detection of antibodies against bovine viral diarrhoea virus (BVDV). The intra- and inter-assay variability are 4.9% and less than 7%, respectively, and variability of bead conjugations is less than 6.6%. The diagnostic performance of the assay was evaluated by testing a total of 509 serum samples. Based on a negative/positive cut-off value of 30.3%, the assay has a sensitivity of 99.4% and a specificity of 98.3% relative to ELISA. The new microsphere immunoassay provides an alternative to conventional ELISA systems and can be used for high-throughput screening in the BVD control programmes. (C) 2010 Elsevier B.V. All rights reserved.
Keywords
Luminex; Microsphere immunoassay; BVDV
Published in
Journal of Virological Methods
2010, volume: 168, number: 1-2, pages: 18-21
Publisher: ELSEVIER SCIENCE BV
SLU Authors
Belak, Sandor
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
UKÄ Subject classification
Animal and Dairy Science
Veterinary Science
Publication identifier
- DOI: https://doi.org/10.1016/j.jviromet.2010.04.009
Permanent link to this page (URI)
https://res.slu.se/id/publ/60460