Abstract

A ring test was completed to determine within laboratory repeatability, and reproducibility among laboratories, of a gas production technique for measuring fermentation kinetics of feeds in rumen fluid. Eight laboratories in different European countries received 20 feed samples. All used the same gas production equipment and method. Gas production profiles were fitted to a multiphasic model and the most important parameters were statistically compared. Although the within laboratory repeatability of, and levels of, calculated parameters differed among laboratories, in most cases there was a high correlation between pairs of laboratories (r = 0.93-1.00), although in some cases the correlation was only moderate (r= 0.73-0.87). Within laboratory gas production profiles were influenced by variation in microbiological activity of the rumen fluid and air pressure changes at the end of the run. Among laboratory differences were observed in calibration factors, type of donor animal and diets fed. Variation sources can be minimized using within run standards. (c) 2005 Elsevier B.V. All rights reserved

Keywords

Gas production; Ranking; Repeatability; Standardization; Variation sources

Published in

Animal Feed Science and Technology
2005, Volume: 123, pages: 243-253
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Hetta, Mårten

  • Department of Agricultural Research for Northern Sweden, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Veterinary Science
Animal and Dairy Science
Agricultural Science


Publication identifier

DOI: https://doi.org/10.1016/j.anifeedsci.2005.04.044

Permanent link to this page (URI)

https://res.slu.se/id/publ/6275