Research article - Peer-reviewed, 2014
Properties of cellular and serum forms of thymidine kinase 1 (TK1) in dogs with acute lymphocytic leukemia (ALL) and canine mammary tumors (CMTs): implications for TK1 as a proliferation biomarker
Jagarlamudi KK, Westberg S, Ronnberg H, Eriksson SAbstract
Conclusions: Serum TK1 protein and activity levels were significantly higher in CMT than in healthy dogs. Size exclusion chromatography demonstrated major differences in the molecular forms of sTK1 in ALL, healthy, and CMT dogs, with a large fraction of inactive TK1 protein in CMT. Our results showed that the sTK1 protein assay can differentiate benign tumors (early stage tumors) from healthy more efficiently than sTK1 activity assay. This preliminary data supports that sTK1 protein assay is clinically useful. Further studies are needed to evaluate the diagnostic or prognostic role of serum TK1 protein in CMTs.Keywords
Canine serum thymidine kinase 1; sTK1 protein assays; Canine mammary tumors; Size exclusion chromatography; Anti-dog TK1 antibodiesPublished in
BMC Veterinary Research2014, volume: 10, article number: 228
Authors' information
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
Westberg, Sara (Saellström, Sara)
Swedish University of Agricultural Sciences, University Animal Hospital
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Anatomy, Physiology and Biochemistry (AFB)
UKÄ Subject classification
Other Veterinary Science
Medical Bioscience
Publication Identifiers
DOI: https://doi.org/10.1186/s12917-014-0228-1
URI (permanent link to this page)
https://res.slu.se/id/publ/64898