Abstract

Metagenomic approaches have become invaluable for culture-independent and sequence-independent detection and characterization of disease-associated pathogens. Here, the sequential steps from sampling to verification of results are described for a metagenomic-based approach to detect potential pathogens in honeybees. The pre-sequencing steps are given in detail, but due to the rapid development of sequencing technologies, all platform-specific procedures, as well as subsequent bioinformatics analysis, are more generally described. It should also be noted that this approach could, with minor modifications, be adapted for other organisms and sample matrices.

Keywords

Virus, metagenomics

Published in

Methods in Molecular Biology
2015, Volume: 1247, number: 1247, pages: 491-511
Title: Veterinary infection biology: molecular diagnostics and high-throughput strategies Methods in Molecular Biology
ISBN: 978-1-4939-2004-4
Publisher: Springer New York

SLU Authors

• Granberg, Fredrik

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Karlsson Lindsjö, Oskar

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Belak, Sandor

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

Associated SLU-program

Future Animal Health and Welfare (until Jan 2017)


UKÄ Subject classification

Microbiology in the medical area


Publication identifier

DOI: https://doi.org/10.1007/978-1-4939-2004-4_33

Permanent link to this page (URI)

https://res.slu.se/id/publ/65802