Comparable constitutive expression and activity of cytochrome P450 between the lobes of the porcine liver

Abstract

Due to limited availability of human liver tissue for the study of cytochrome P450 (CYP450), porcine liver tissue has been suggested as an alternative source to prepare microsomes and hepatocytes. The porcine liver is made by four different lobes. The present study investigated the expression and activity of specific CYP450 isoforms in the four lobes, with the purpose to examine if one lobe of the porcine liver resembles the human more than others. Samples from the four major lobes were taken from female pigs and mRNA expression and activity of CYP1A, 2A, 2C, 2D, 2E and 3A determined. The results showed no differences in specific mRNA expression and activity of any of the investigated CYP450 isoforms. In conclusion, the study shows that all parts of the porcine liver are equally useful as model tissue. (C) 2014 Elsevier Ltd. All rights reserved.

Keywords

Porcine liver; Model system; Detoxification; PCA; Inter-individual; Intra-hepatic

Published in

Toxicology in Vitro
2014, volume: 28, number: 7, pages: 1190-1195
Publisher: PERGAMON-ELSEVIER SCIENCE LTD

SLU Authors

• Zamaratskaia, Galia

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology

Publication identifier

• DOI: https://doi.org/10.1016/j.tiv.2014.06.002

Permanent link to this page (URI)

https://res.slu.se/id/publ/66190