Sensitive detection of aggregated prion protein via proximity ligation

Abstract

The DNA assisted solid-phase proximity ligation assay (SP-PLA) provides a unique opportunity to specifically detect prion protein (PrP) aggregates by investigating the collocation of 3 or more copies of the specific protein. We have developed an SP-PLA that can detect PrP aggregates in brain homogenates from infected hamsters even after a 10(7)-fold dilution. In contrast, brain homogenate from uninfected animals did not generate a detectable signal at 100-fold higher concentration. Using either of the 2 monoclonal anti-PrP antibodies, 3F4 and 6H4, we successfully detected low concentrations of aggregated PrP. The presented results provide a proof of concept that this method might be an interesting tool in the development of diagnostic approaches of prion diseases.

Keywords

diagnosis; monoclonal antibody; prion protein; proximity ligation assay; 263K

Published in

Prion
2014, volume: 8, number: 3, pages: 261-265
Publisher: LANDES BIOSCIENCE

SLU Authors

• Linne, Tommy

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry
Molecular Biology

Publication identifier

• DOI: https://doi.org/10.4161/pri.32231

Permanent link to this page (URI)

https://res.slu.se/id/publ/67818