NMR Study of the O-Specific Polysaccharide and the Core Oligosaccharide from the Lipopolysaccharide Produced by Plesiomonas shigelloides O24:H8 (Strain CNCTC 92/89)

Lundqvist, Lena; Kaszowska, Marta; Sandström, Corine

doi:10.3390/molecules20045729

Research article - Peer-reviewed, 2015

NMR Study of the O-Specific Polysaccharide and the Core Oligosaccharide from the Lipopolysaccharide Produced by Plesiomonas shigelloides O24:H8 (Strain CNCTC 92/89)

Lundqvist, Lena; Kaszowska, Marta; Sandström, Corine

Abstract

The structures of the O-specific polysacccharide and core oligosaccharide of the lipopolysaccharide from Plesiomonas shigelloides O24:H8, strain CNCTC 92/89, have been investigated by NMR spectroscopy and ESI mass spectrometry. The O-specific polysaccharide was found to be composed of a tetrasaccharide repeating unit consisting of [3)--FucpNAc-(13)--GalpNAcA-(13)--QuipNAc-(1] and of -RhapNAc (14) linked to the GalpNAcA residue. An identical structure has been reported for the capsular polysaccharide of the clinical isolate of Vibrio vulnificus strain BO62316 [1]. The core oligosaccharide was composed of a decasaccharide which structure is identical with these in P. shigelloides serotype O54 [2] and serotype O37 [3].

Keywords

lipopolysaccharide; Plesiomonas shigelloides; NMR; ESI MS; structural analysis

Published in

Molecules
2015, volume: 20, number: 4, pages: 5729-5739
Publisher: MDPI AG

Authors' information

Lundqvist, Lena

Swedish University of Agricultural Sciences, Department of Chemistry

Kaszowska, Marta

Polish Academy of Sciences (PAN)

Sandström, Corine

Swedish University of Agricultural Sciences, The Department of Chemistry and Biotechnology

UKÄ Subject classification

Analytical Chemistry

Publication Identifiers

DOI: https://doi.org/10.3390/molecules20045729

URI (permanent link to this page)

https://res.slu.se/id/publ/69714