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Research article - Peer-reviewed, 2003

High-performance liquid chromatographic analysis of secoisolariciresinol diglucoside and hydroxycinnamic acid glucosides in flaxseed by alkaline extraction

Eliasson C, Kamal-Eldin A, Andersson R, Aman P

Abstract

A HPLC method was developed for the analysis of secoisolariciresinol diglucoside (SDG) and hydroxycinnamic acid glucosides in milled defatted flaxseed flour. Direct extraction by I M NaOH for I h at 20 degreesC resulted in a higher yield than that obtained by hydrolysis of alcoholic extracts. An internal standard, o-coumaric acid, was used and the method was found to be easy, fast, and with good repeatability. On dry matter basis, different samples of flaxseeds varied considerably in their content of (+)-SDG (11.9-25.9 mg/g), (-)-SDG (2.2-5.0 mg/g), p-coumaric acid glucoside (1.2-8.5 mg/g), and ferulic acid glucoside (1.6-5.0 mg/g). (C) 2003 Published by Elsevier B.V

Published in

Journal of Chromatography A
2003, volume: 1012, number: 2, pages: 151-159
Publisher: ELSEVIER SCIENCE BV

Authors' information

Swedish University of Agricultural Sciences, Department of Food Science
Swedish University of Agricultural Sciences, Department of Food Science
Kamal-Eldin, Afaf
Swedish University of Agricultural Sciences, Department of Food Science
Eliasson, Christina
Swedish University of Agricultural Sciences, Department of Food Science

UKÄ Subject classification

Food Science

Publication Identifiers

DOI: https://doi.org/10.1016/S0021-9673(03)01136-1

URI (permanent link to this page)

https://res.slu.se/id/publ/711