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Research article2005Peer reviewed

RAPD-based analysis of genetic diversity and selection of lingonberry (Vaccinium vitis-idaea L.) material for ex situ conservation

Garkava-Gustavsson L, Persson HA, Nybom H, Rumpunen K, Gustavsson BA, Bartish IV

Abstract

Random amplified polymorphic DNA markers were used to assess relatedness and genetic diversity for 15 lingonberry (Vaccinium vitis-idaea) populations. Seven primers yielding 59 polymorphic bands were used to analyse 13 populations, representing ssp. vitis-idaea from Sweden, Finland, Norway, Estonia and Russia, and two populations, representing ssp. minus from Japan and Canada. A cluster analysis and a multidimensional scaling analysis (MDS) showed similar phenetic patterns among populations, with a pronounced geographic grouping in most cases. Significant correlations were obtained between geographic and genetic distances for the entire set of populations as well as for the 13 ssp. vitis-idaea populations. Mean within-population diversity was 0.206 when estimated with Lynch and Milligan's index, and 0.431 when estimated with Shannon's index, which is in agreement with the mixed mating system reported for lingonberry. Within-population variability accounted for 68.6% of the total variance when all populations were included, and for 78.8% when only populations of ssp. vitis-idaea were analysed. Two different approaches were applied to the selection of plant material for a potential gene bank: (1) a hierarchical sampling strategy based on a cluster analysis and (2) the Maximum genetic diversity program, developed for the establishment of core collections. Random sampling was undertaken for comparisons with the selected data sets. The most diverse and representative set of lingonberry specimens was obtained when samples were selected with the Maximum diversity program

Published in

Genetic Resources and Crop Evolution
2005, Volume: 52, number: 6, pages: 723-735
Publisher: SPRINGER

      SLU Authors

    • Gustavsson, Larisa

      • Department of Crop Science, Swedish University of Agricultural Sciences

        • Nybom, Hilde

          • Department of Crop Science, Swedish University of Agricultural Sciences

          • Rumpunen, Kimmo

            • Department of Crop Science, Swedish University of Agricultural Sciences

            • Gustavsson, Björn

              • Department of Crop Science, Swedish University of Agricultural Sciences

              • Bartish, Igor

                • Department of Crop Science, Swedish University of Agricultural Sciences

              UKÄ Subject classification

              Horticulture

              Publication identifier

              DOI: https://doi.org/10.1007/s10722-003-6123-4

              Permanent link to this page (URI)

              https://res.slu.se/id/publ/7216