Abstract

The apoplast can be described as the soluble fraction of the extracellular space of plant tissue, and it plays an important role in signaling, nutrient transport, and plant-pathogen interactions. In this protocol, we describe a method where leaves are infiltrated with phosphate buffer under vacuum. The apoplast can then be extracted by centrifugation and simultaneously collected in a protease inhibitor solution. Using this protocol, typically 3 mu g of apoplastic proteins can be obtained in a volume of 300 mu L from five potato leaflets, with minimal contamination by non-apoplastic proteins.

Keywords

Apoplast; Apoplastic protein; Secretome; Soluble fraction; Protease inhibitor; Phosphate buffer; Vacuum infiltration; Extracellular space

Published in

Methods in Molecular Biology
2017, Volume: 1511, number: 1511, pages: 233-240
Title: Isolation of Plant Organelles and Structures
ISBN: 978-1-4939-6531-1
Publisher: Springer

SLU Authors

• Andreasson, Erik

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Abreha, Kibrom

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

• Resjö, Svante

  • Department of Plant Protection Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1007/978-1-4939-6533-5_18

Permanent link to this page (URI)

https://res.slu.se/id/publ/77554