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Research article - Peer-reviewed, 2016

Genetic diversity and occurrence of the F129L substitutions among isolates of Alternaria solani in south-eastern Sweden

Odilbekov, Firuz; Edin, Eva; Gustavsson, Larisa; Persson Hovmalm, Helena; Liljeroth, Erland

Abstract

Background: Early blight, caused by the fungus Alternaria solani, occurs on potato mainly in the south-eastern part of Sweden, but also in other parts of the country. The aim of this study was to investigate the genetic diversity of A. solani populations from different potato growing regions in south-eastern Sweden using AFLP marker analysis. In addition, the cultured isolates were examined for substitutions in the gene encoding cytochrome b, associated with loss of sensitivity against QoI fungicides.Results: Nei's gene diversity index for the Swedish populations of A. solani revealed a gene diversity of up to 0.20. Also genetic differentiation was observed among populations of A. solani from different locations in south-eastern Sweden. The mitochondrial genotype of the isolates of A. solani was determined and both known genotypes, GI (genotype 1) and GII (genotype 2), were found among the isolates. The occurrence of the F129L substitution associated with a loss of sensitivity to strobilurins was confirmed among the GII isolates. In vitro conidial germination tests verified that isolates containing the F129L substitution had reduced sensitivity to azoxystrobin and, at a lower extent, to pyraclostrobin.Conclusions: Genetic diversity was relatively high among isolates of A. solani in south-eastern part of Sweden. F129L substitutions, leading to reduced sensitivity to strobilurins, have been established in field populations, which may have implications for the future efficacy of QoI fungicides.

Keywords

AFLP; Early blight; Potato; Strobilurin; Sensitivity

Published in

Hereditas
2016, volume: 153, article number: 10
Publisher: BIOMED CENTRAL LTD

Authors' information

Edin, Eva
Swedish University of Agricultural Sciences, Department of Forest Mycology and Plant Pathology
Swedish University of Agricultural Sciences, Department of Plant Breeding
Swedish University of Agricultural Sciences, Department of Plant Breeding
Swedish University of Agricultural Sciences, Department of Plant Protection Biology
Swedish University of Agricultural Sciences, Department of Plant Protection Biology

Associated SLU-program

SLU Network Plant Protection

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.1186/s41065-016-0014-0

URI (permanent link to this page)

https://res.slu.se/id/publ/77587