Abstract

In this study we evaluated and applied an HPLC coulometric array (HPLC-CoulArray) method for simultaneous determination of flavonols and phenolic acids in bilberry, lingonberry, cloudberry and sea buckthorn berry. Berry samples purchased from local supermarkets were freeze-dried, hydrolysed and extracted with 50% aqueous methanol containing TBHQ antioxidant and 1.2 M HCL, and analysed with HPLC-CoulArray. We found that phenolic profiles differed between berries. Quercetin was found in almost all berries, with the highest concentrations observed in lingonberry. Myricetin and isorhamnetin were only detected in bilberry and sea-buckthorn, respectively. Hydroxycinnamic acids (caffeic, ferulic, p-coumaric) were also detected in all berries, but hydroxybenzoic acids (gallic, vanillic) were not found in sea-buckthorn. Total concentration of phenolic compounds was highest in sea-buckthorn berry (270.5 mg/100 g DW), followed by bilberry (253.6 mg/100 g DW), lingonberry (219.7 mg/100 g DW) and cloudberry (121.7 mg/100 g DW). (C) 2016 Elsevier Ltd. All rights reserved.

Keywords

Bilberry; Lingonberry; Cloudberry; Sea-buckthorn; Phenolic compounds

Published in

Food Science and Technology
2016, Volume: 74, pages: 128-134
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Zamaratskaia, Galia

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    

• Landberg, Rikard

  • Department of Molecular Sciences, Swedish University of Agricultural Sciences
    
  • Karolinska Institute

UKÄ Subject classification

Analytical Chemistry


Publication identifier

DOI: https://doi.org/10.1016/j.lwt.2016.07.034

Permanent link to this page (URI)

https://res.slu.se/id/publ/77970