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Research article2003Peer reviewed

Crystal structure of naphthalene dioxygenase: Side-on binding of dioxygen to iron

Karlsson A, Parales JV, Parales RE, Gibson DT, Eklund H, Ramaswamy S


Binding of oxygen to iron is exploited in several biological and chemical processes. Although computational and spectroscopic results have suggested side-on binding, only end-on binding of oxygen to iron has been observed in crystal structures. We have determined structures of naphthalene dioxygenase that show a molecular oxygen species bound to the mononuclear iron in a side-on fashion. In a complex with substrate and dioxygen, the dioxygen molecule is lined up for an attack on the double bond of the aromatic substrate. The structures reported here provide the basis for a reaction mechanism and for the high stereospecificity of the reaction catalyzed by naphthalene dioxygenase

Published in

2003, Volume: 299, number: 5609, pages: 1039-1042

      SLU Authors

      • Eklund, Hans

        • Department of Molecular Biology, Swedish University of Agricultural Sciences

      UKÄ Subject classification

      Environmental Sciences related to Agriculture and Land-use

      Publication identifier


      Permanent link to this page (URI)