Karlsson Tiselius, Andreas
- Department of Molecular Biology, Swedish University of Agricultural Sciences
Research article2003Peer reviewed
Karlsson, A; Parales, JV; Parales, RE; Gibson, DT; Eklund, H; Ramaswamy, S
Binding of oxygen to iron is exploited in several biological and chemical processes. Although computational and spectroscopic results have suggested side-on binding, only end-on binding of oxygen to iron has been observed in crystal structures. We have determined structures of naphthalene dioxygenase that show a molecular oxygen species bound to the mononuclear iron in a side-on fashion. In a complex with substrate and dioxygen, the dioxygen molecule is lined up for an attack on the double bond of the aromatic substrate. The structures reported here provide the basis for a reaction mechanism and for the high stereospecificity of the reaction catalyzed by naphthalene dioxygenase
Science
2003, volume: 299, number: 5609, pages: 1039-1042
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Environmental Sciences and Nature Conservation
https://res.slu.se/id/publ/796