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Research article2003Peer reviewed

Crystal structure of naphthalene dioxygenase: Side-on binding of dioxygen to iron

Karlsson, A; Parales, JV; Parales, RE; Gibson, DT; Eklund, H; Ramaswamy, S

Abstract

Binding of oxygen to iron is exploited in several biological and chemical processes. Although computational and spectroscopic results have suggested side-on binding, only end-on binding of oxygen to iron has been observed in crystal structures. We have determined structures of naphthalene dioxygenase that show a molecular oxygen species bound to the mononuclear iron in a side-on fashion. In a complex with substrate and dioxygen, the dioxygen molecule is lined up for an attack on the double bond of the aromatic substrate. The structures reported here provide the basis for a reaction mechanism and for the high stereospecificity of the reaction catalyzed by naphthalene dioxygenase

Published in

Science
2003, volume: 299, number: 5609, pages: 1039-1042
Publisher: AMER ASSOC ADVANCEMENT SCIENCE

SLU Authors

  • Karlsson Tiselius, Andreas

    • Department of Molecular Biology, Swedish University of Agricultural Sciences

  • Eklund, Hans

    • Department of Molecular Biology, Swedish University of Agricultural Sciences

UKÄ Subject classification

Environmental Sciences and Nature Conservation

Publication identifier

  • DOI: https://doi.org/10.1126/science.1078020

Permanent link to this page (URI)

https://res.slu.se/id/publ/796