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Research article - Peer-reviewed, 2003

Crystal structure of naphthalene dioxygenase: Side-on binding of dioxygen to iron

Karlsson A, Parales JV, Parales RE, Gibson DT, Eklund H, Ramaswamy S

Abstract

Binding of oxygen to iron is exploited in several biological and chemical processes. Although computational and spectroscopic results have suggested side-on binding, only end-on binding of oxygen to iron has been observed in crystal structures. We have determined structures of naphthalene dioxygenase that show a molecular oxygen species bound to the mononuclear iron in a side-on fashion. In a complex with substrate and dioxygen, the dioxygen molecule is lined up for an attack on the double bond of the aromatic substrate. The structures reported here provide the basis for a reaction mechanism and for the high stereospecificity of the reaction catalyzed by naphthalene dioxygenase

Published in

Science
2003, volume: 299, number: 5609, pages: 1039-1042
Publisher: AMER ASSOC ADVANCEMENT SCIENCE

Authors' information

Karlsson, Andreas (Karlsson Tiselius, Andreas)
Swedish University of Agricultural Sciences, Department of Molecular Biology
Eklund, Hans
Swedish University of Agricultural Sciences, Department of Molecular Biology
Gibson, David T
Parales, Rebecca E
Parales, Juanito V
Ramaswamy, S
Swedish University of Agricultural Sciences, Department of Molecular Biology

UKÄ Subject classification

Environmental Sciences related to Agriculture and Land-use

Publication Identifiers

DOI: https://doi.org/10.1126/science.1078020

URI (permanent link to this page)

https://res.slu.se/id/publ/796