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Research article - Peer-reviewed, 2003

Crystal structure of naphthalene dioxygenase: Side-on binding of dioxygen to iron

Karlsson A, Parales JV, Parales RE, Gibson DT, Eklund H, Ramaswamy S

Abstract

Binding of oxygen to iron is exploited in several biological and chemical processes. Although computational and spectroscopic results have suggested side-on binding, only end-on binding of oxygen to iron has been observed in crystal structures. We have determined structures of naphthalene dioxygenase that show a molecular oxygen species bound to the mononuclear iron in a side-on fashion. In a complex with substrate and dioxygen, the dioxygen molecule is lined up for an attack on the double bond of the aromatic substrate. The structures reported here provide the basis for a reaction mechanism and for the high stereospecificity of the reaction catalyzed by naphthalene dioxygenase

Published in

Science
2003, Volume: 299, number: 5609, pages: 1039-1042
Publisher: AMER ASSOC ADVANCEMENT SCIENCE

      SLU Authors

    • Karlsson Tiselius, Andreas

      • Department of Molecular Biology, Swedish University of Agricultural Sciences

      • Eklund, Hans

        • Department of Molecular Biology, Swedish University of Agricultural Sciences

      UKÄ Subject classification

      Environmental Sciences related to Agriculture and Land-use

      Publication Identifiers

      DOI: https://doi.org/10.1126/science.1078020

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/796