Skip to main content
SLU:s publikationsdatabas (SLUpub)

Forskningsartikel1993Vetenskapligt granskad

Classification of Embryogenic Cell-lines of Picea abies as Regards Protoplast Isolation and Culture

Egertsdotter, Ulrika; Von Arnold, Sara

Sammanfattning

Embryogenic cell-lines of Picea abies were divided into two groups based on their growth habit and the morphology of the somatic embryos. Group A grew well on solid medium and the head regions of the somatic embryos were dense, while group B grew well in suspension and the head region of the somatic embryos consisted of loosely packed cells.Protoplasts from all cell-lines could divide and enter the embryogenic pathway. The highest proportion of dividing cells entering the embryogenic pathway was obtained from cell-lines belonging to group A cultured on solid medium. However, only the protoplast derived colonies from cell-lines belonging to group B growing as suspensions continued to grow and gave rise to many somatic embryos.New embryogenic suspension cultures were established from protoplasts of one cell-line belonging to group B. Growth habit and embryo morphology were similar for the re-established suspension and the initial culture.

Nyckelord

SOMATIC EMBRYOGENESIS; EMBRYOGENIC CELL-LINES; NORWAY SPRUCE; PICEA-ABIES; PROTOPLAST REGENERATION

Publicerad i

Journal of Plant Physiology
1993, Volym: 141, nummer: 2, sidor: 222-229
Utgivare: GUSTAV FISCHER VERLAG

      SLU författare

      • Von Arnold, Sara

        • Institutionen för skogsgenetik, Sveriges lantbruksuniversitet

      UKÄ forskningsämne

      Cellbiologi

      Publikationens identifierare

      DOI: https://doi.org/10.1016/S0176-1617(11)80764-9

      Permanent länk till denna sida (URI)

      https://res.slu.se/id/publ/79934