Biomimetic spinning of artificial spider silk from a chimeric minispidroin

Abstract

Herein we present a chimeric recombinant spider silk protein (spidroin) whose aqueous solubility equals that of native spider silk dope and a spinning device that is based solely on aqueous buffers, shear forces and lowered pH. The process recapitulates the complex molecular mechanisms that dictate native spider silk spinning and is highly efficient; spidroin from one liter of bacterial shake-flask culture is enough to spin a kilometer of the hitherto toughest as-spun artificial spider silk fiber.

Published in

Nature Chemical Biology
2017, Volume: 13, number: 3, pages: 262-264
Publisher: NATURE PUBLISHING GROUP

SLU Authors

• Andersson, Marlene

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Johansson, Jan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • Karolinska Institute

• Rising, Anna

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • Karolinska Institute

UKÄ Subject classification

Structural Biology
Biochemistry and Molecular Biology
Biomaterials Science


Publication identifier

DOI: https://doi.org/10.1038/NCHEMBIO.2269

Permanent link to this page (URI)

https://res.slu.se/id/publ/80753