Skip to main content
Research article - Peer-reviewed, 2016

A new 2DS center dot 2RL Robertsonian translocation transfers stem rust resistance gene Sr59 into wheat

Rahmatov, Mahbubjon; Rouse, Matthew; Nirmala, Jayaveeramuthu; Danilova, Tatiana; Friebe, Bernd; Steffenson, Brian; Johansson, Eva

Abstract

Key message A new stem rust resistance gene Sr59 from Secale cereale was introgressed into wheat as a 2DS center dot 2RL Robertsonian translocation.Abstract Emerging new races of the wheat stem rust pathogen (Puccinia graminis f. sp. tritici), from Africa threaten global wheat (Triticum aestivum L.) production. To broaden the resistance spectrum of wheat to these widely virulent African races, additional resistance genes must be identified from all possible gene pools. From the screening of a collection of wheat-rye (Secale cereale L.) chromosome substitution lines developed at the Swedish University of Agricultural Sciences, we described the line 'SLU238' 2R (2D) as possessing resistance to many races of P. graminis f. sp. tritici, including the widely virulent race TTKSK (isolate synonym Ug99) from Africa. The breakage-fusion mechanism of univalent chromosomes was used to produce a new Robertsonian translocation: T2DS.2RL. Molecular marker analysis and stem rust seedling assays at multiple generations confirmed that the stem rust resistance from 'SLU238' is present on the rye chromosome arm 2RL. Line TA5094 (#101) was derived from 'SLU238' and was found to be homozygous for the T2DS.2RL translocation. The stem rust resistance gene on chromosome 2RL arm was designated as Sr59. Although introgressions of rye chromosome arms into wheat have most often been facilitated by irradiation, this study highlights the utility of the breakage-fusion mechanism for rye chromatin introgression. Sr59 provides an additional asset for wheat improvement to mitigate yield losses caused by stem rust.

Published in

TAG Theoretical and Applied Genetics
2016, volume: 129, number: 7, pages: 1383-1392
Publisher: SPRINGER

Authors' information

Swedish University of Agricultural Sciences, Department of Plant Breeding
University of Minnesota
Tajik Agrarian University Shirinsho Shotemur
Rouse, Matthew
United States Department of Agriculture (USDA)
Nirmala, Jayaveeramuthu
United States Department of Agriculture (USDA)
Danilova, Tatiana
Kansas State University
Friebe, Bernd
Kansas State University
Steffenson, Brian
University of Minnesota
Swedish University of Agricultural Sciences, Department of Plant Breeding

Associated SLU-program

SLU Network Plant Protection

UKÄ Subject classification

Agricultural Science

Publication Identifiers

DOI: https://doi.org/10.1007/s00122-016-2710-6

URI (permanent link to this page)

https://res.slu.se/id/publ/80922