Skip to main content
SLU:s publikationsdatabas (SLUpub)

Forskningsartikel2016Vetenskapligt granskadÖppen tillgång

The Large Subunit rDNA Sequence of Plasmodiophora brassicae Does not Contain Intra-species Polymorphism

Schwelm, Arne; Berney, Cédric; Dixelius, Christina; Bass, David; Neuhauser, Sigrid

Sammanfattning

Clubroot disease caused by Plasmodiophora brassicae is one of the most important diseases of cultivated brassicas. P. brassicae occurs in pathotypes which differ in the aggressiveness towards their Brassica host plants. To date no DNA based method to distinguish these pathotypes has been described. In 2011 polymorphism within the 28S rDNA of P. brassicae was reported which potentially could allow to distinguish pathotypes without the need of time-consuming bioassays. However, isolates of P. brassicae from around the world analysed in this study do not show polymorphism in their LSU rDNA sequences. The previously described polymorphism most likely derived from soil inhabiting Cercozoa more specifically Neoheteromita-like glissomonads. Here we correct the LSU rDNA sequence of P. brassicae. By using FISH we demonstrate that our newly generated sequence belongs to the causal agent of clubroot disease. (C) 2016 The Authors. Published by Elsevier GmbH.

Nyckelord

Clubroot; FISH (fluorescence in-situ hybridisation); LSU rDNA; pathotyping; phytomyxea; plasmodiophorids

Publicerad i

Protist
2016, Volym: 167, nummer: 6, sidor: 544-554

      SLU författare

    • UKÄ forskningsämne

      Strukturbiologi
      Utvecklingsbiologi
      Cellbiologi

      Publikationens identifierare

      DOI: https://doi.org/10.1016/j.protis.2016.08.008

      Permanent länk till denna sida (URI)

      https://res.slu.se/id/publ/81031