Geladi, Paul
- Department of Forest Biomaterials and Technology, Swedish University of Agricultural Sciences
- Corpus Data & Image Analysis AB
Research article2017Peer reviewed
Detection of low levels of Escherichia coli by electrochemical impedance spectroscopy and singular value decomposition
Krupinska, Karolina; Geladi, Paul; Vikholm-Lundin, Inger; Lindholm-Sethson, Britta
Abstract
The first steps are reported in the development of a new ultrasensitive electrochemical biosensor for detection of Escherichia colt (E. colt) in water. Two gold electrodes in a sandwich flow-cell were modified with E. coli polyclonal antibody and exposed to three different concentrations of E. coll. Electrochemical Impedance Spectroscopy was used in combination with Singular Value Decompostion of the complex numbers to monitor the interactions at the electrode surfaces. A linear regression line in the concentration range 10-1000 CFU*m1(-1) was obtained without use of redox probes or metal nanoparticles for signal amplification.
Published in
Chemometrics and Intelligent Laboratory Systems
2017, Volume: 163, pages: 49-54
Publisher: ELSEVIER SCIENCE BV
SLU Authors
UKÄ Subject classification
Analytical Chemistry
Publication identifier
DOI: https://doi.org/10.1016/j.chemolab.2016.11.008
Permanent link to this page (URI)
https://res.slu.se/id/publ/82740