Silk matrices promote formation of insulin-secreting islet-like clusters

Sammanfattning

Ex vivo expansion of endocrine cells constitutes an interesting alternative to be able to match the unmet need of transplantable pancreatic islets. However, endocrine cells become fragile once removed from their extracellular matrix (ECM) and typically become senescent and loose insulin expression during conventional 2D culture. Herein we develop a protocol where 3D silk matrices functionalized with ECM derived motifs are used for generation of insulin-secreting islet-like clusters from mouse and human primary cells. The obtained clusters were shown to attain an islet-like spheroid shape and to maintain functional insulin release upon glucose stimulation in vitro. Furthermore, in vivo imaging of transplanted murine clusters showed engraftment with increasing vessel formation during time. There was no sign of cell death and the clusters maintained or increased in size throughout the period, thus suggesting a suitable cluster size for transplantation. (C) 2016 Elsevier Ltd. All rights reserved.

Nyckelord

Recombinant spider silk; Pancreatic islets; Islet-like clusters; Insulin release; In vivo imaging; Vascularization

Publicerad i

Biomaterials
2016, Volym: 90, sidor: 50-61
Utgivare: ELSEVIER SCI LTD

SLU författare

• Hedhammar, My

  • Institutionen för husdjurens biovetenskaper, Sveriges lantbruksuniversitet
    
  • Kungliga Tekniska Högskolan (KTH)

Globala målen

SDG3 God hälsa och välbefinnande


UKÄ forskningsämne

Biomaterialvetenskap


Publikationens identifierare

DOI: https://doi.org/10.1016/j.biomaterials.2016.03.006

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/82883