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Forskningsartikel - Refereegranskat, 2016

NMR-based metabolomics reveals compartmental metabolic heterogeneity in liver of Arctic char (Salvelinus alpinus)

Cheng, K.; Wagner, L.; Pickova, J.; Moazzami, A. A.

Sammanfattning

Metabolomics involves systematic study of low-molecular-mass metabolites in cells, tissues, or biofluids and is nowadays widely applied to characterize the physiological status of aquatic organisms under a set of conditions, such as disease and toxin exposure. Liver, an important metabolic center in the fish body, is often used for metabolomics analysis. Compared with the whole fish liver, the proportion of liver sample needed for metabolomics analysis is relatively small. The homogeneity of metabolites in liver is thus an important issue, especially for comparative studies and biomarker discovery. This study examined the homogeneity of the metabolic profile in liver of Arctic char (Salvelinus alpinus (L., 1758)) using a NMR-based metabolomics approach. For the analysis, whole liver samples were cut into four parts along the direction of gall bladder and at right angles to this, and metabolites in each part of the liver were extracted and analyzed by multivariate and univariate data analyses. Although the multivariate model was not significant due to variation within the data, the metabolic differences in polar portion of liver extract between the parts were seen, indicating non-homogeneity of Arctic char liver. Therefore, when sampling fish liver for further metabolomics studies, this heterogeneity should be taken into consideration.

Nyckelord

anatomic compartment; Arctic char; liver; NMR-based metabolomics; Salvelinus alpinus

Publicerad i

Canadian Journal of Zoology
2016, Volym: 94, nummer: 9, sidor: 665-669
Utgivare: CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS