Abstract

In order to investigate the potential of magic angle spinning nuclear magnetic resonance (MAS NMR) in the elucidation of post-mortem metabolism in muscle biopsies, simultaneous H-1 and (31)p MAS NMR measurements were made continuously on postmortem (20 min to 24 h) muscle longissimus samples from rabbits. The animals had either been or not been given adrenaline (0.5 mg kg(-1) 4 h pre-slaughter) to deplete stores of muscle glycogen. The intracellular pH was calculated from H-1 spectra, and the post-mortem rate of formation of lactate was followed and quantified. Comparison of measurements made on muscle samples from rabbits treated with adrenaline with measurements made on muscle samples from untreated' rabbits revealed significant effects of adrenaline treatment on both pH (pH24 h = 6.42 vs. pH24 It = 5.60) and formation of lactate (16 mmol g(-1) vs. 65 mmol g(-1)). The P-31 NMR spectra were used to follow the rate of degradation of ATP and phosphocreatine. The present study clearly shows that MAS NMR has potential for the study of post-mortem energy metabolism.

Keywords

glycogen status; lactate; magic angle spinning; NMR; pH; phosphorus metabolites; water-holding capacity

Published in

International Journal of Food Science and Technology
2004, Volume: 39, number: 6, pages: 661-670
Publisher: BLACKWELL PUBLISHING LTD

UKÄ Subject classification

Food Science


Publication identifier

DOI: https://doi.org/10.1111/j.1365-2621.2004.00826.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/85647