Andersson, Mariette
- Institutionen för växtförädling, Sveriges lantbruksuniversitet
Forskningsartikel2017Vetenskapligt granskadÖppen tillgång
Iskandarov, Umidjon; Silva, Jillian E.; Kim, Hae Jin; Andersson, Mariette; Cahoon, Rebecca E.; Mockaitis, Keithanne; Cahoon, Edgar B.
Seed oils of many Cuphea sp. contain.90% of medium-chain fatty acids, such as decanoic acid (10: 0). These seed oils, which are among the most compositionally variant in the plant kingdom, arise from specialized fatty acid biosynthetic enzymes and specialized acyltransferases. These include lysophosphatidic acid acyltransferases (LPAT) and diacylglycerol acyltransferases (DGAT) that are required for successive acylation of medium-chain fatty acids in the sn-2 and sn-3 positions of seed triacylglycerols (TAGs). Here we report the identification of a cDNA for a DGAT1-type enzyme, designated CpuDGAT1, from the transcriptome of C. avigera var pulcherrima developing seeds. Microsomes of camelina (Camelina sativa) seeds engineered for CpuDGAT1 expression displayed DGAT activity with 10:0-CoA and the diacylglycerol didecanoyl, that was approximately 4-fold higher than that in camelina seed microsomes lacking CpuDGAT1. In addition, coexpression in camelina seeds of CpuDGAT1 with a C. viscosissima FatB thioesterase (CvFatB1) that generates 10:0 resulted in TAGs with nearly 15 mol % of 10: 0. More strikingly, expression of CpuDGAT1 and CvFatB1 with the previously described CvLPAT2, a 10:0-CoA-specific Cuphea LPAT, increased 10:0 amounts to 25 mol % in camelina seed TAG. These TAGs contained up to 40 mol % 10:0 in the sn-2 position, nearly double the amounts obtained from coexpression of CvFatB1 and CvLPAT2 alone. Although enriched in diacylglycerol, 10:0 was not detected in phosphatidylcholine in these seeds. These findings are consistent with channeling of 10:0 into TAG through the combined activities of specialized LPAT and DGAT activities and demonstrate the biotechnological use of these enzymes to generate 10:0-rich seed oils.
Plant Physiology
2017, Volym: 174, nummer: 1, sidor: 97-109 Utgivare: AMER SOC PLANT BIOLOGISTS
Växtbioteknologi
DOI: https://doi.org/10.1104/pp.16.01894
https://res.slu.se/id/publ/87613