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Forskningsartikel1998Vetenskapligt granskadÖppen tillgång

Development of somatic embryos in Norway spruce

Egertsdotter, U; von Arnold, S


Embryogenic cell lines of Norway spruce consist of a large number of somatic embryos. The cell lines have been divided into two groups, A and B. The group B embryos are developmentally blocked, Extracts of mature spruce seeds stimulate group B embryos to develop a morphology comparable to group A embryos. However, seed extract inhibits early embryo development. The active components in seed extract were shown probably to be proteins. Extracts of mature seeds contain chitinase-like proteins as recognized by an antibody towards chitinase 4 in sugar beet. Proteins of similar sizes were detected by the same antibody in the conditioned medium of group A, but not in group B. A chitinase cl-related chitinase and a nod factor had a stimulating effect on early embryo development, but did not influence the later stages of embryo development.


Picea abies; somatic embryogenesis; embryo development; seed extract

Publicerad i

Journal of Experimental Botany
1998, Volym: 49, nummer: 319, sidor: 155-162

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