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Research article2006Peer reviewedOpen access

U1-like snRNAs lacking complementarity to canonical 5 ' splice sites

Kyriakopoulou C, Larsson P, Liu L, Schuster J, Soderbom F, Kirsebom LA, Virtanen A

Abstract

We have detected a surprising heterogeneity among human spliceosomal U1 small nuclear RNA ( snRNA). Most interestingly, we have identified three U1 snRNA variants that lack complementarity to the canonical 59 splice site (5'SS) GU dinucleotide. Furthermore, we have observed heterogeneity among the identified variant U1 snRNA genes caused by single nucleotide polymorphism ( SNP). The identified snRNAs were ubiquitously expressed in a variety of human tissues representing different stages of development and displayed features of functional spliceosomal snRNAs, i.e., trimethylated cap structures, association with Sm proteins and presence in nuclear RNA-protein complexes. The unanticipated heterogeneity among spliceosomal snRNAs could contribute to the complexity of vertebrates by expanding the coding capacity of their genomes

Published in

RNA
2006, volume: 12, number: 9, pages: 1603-1611
Publisher: COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT

SLU Authors

  • Söderbom, Fredrik

    • Department of Molecular Biology, Swedish University of Agricultural Sciences

Publication identifier

  • DOI: https://doi.org/10.1261/rna.26506

Permanent link to this page (URI)

https://res.slu.se/id/publ/8912