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Översiktsartikel - Refereegranskat, 2000

Multiple cyclin-dependent kinase complexes and phosphatases control G(2)/M progression in alfalfa cells

Meszaros, T; Miskolczi, P; Ayaydin, F; Pettko-Szandtner, A; Peres, A; Magyar, Z; Horvath, GV; Bako, L; Feher, A; Dudits, D

Sammanfattning

Reversible phosphorylation of proteins by kinases and phosphatases plays a key regulatory role in several eukaryotic cellular functions including the control of the division cycle. Increasing numbers of sequence and biochemical data show the involvement of cyclin-dependent kinases (CDKs) and cyclins in regulation of the cell cycle progression in higher plants. The complexity represented by different types of CDKs and cyclins in a single species such as alfalfa, indicates that multicomponent regulatory pathways control G(2)/M transition. A set of cdc2-related genes (cdc2Ms A, B, D and F) was expressed in G(2) and M cells. Phosphorylation assays also revealed that at least three kinase complexes (Cdc2Ms A/B, D and F) were successively active in G(2)/M cells after synchronization. Interaction between alfalfa mitotic cyclin (Medsa;CycB2;1) and a kinase partner has been reported previously. The present yeast two-hybrid analyses showed differential interaction between defined D-type cyclins and Cdc2Ms kinases functioning in G(2)/M phases. Localization of Cdc2Ms F kinase to the preprophase band (PPB), the perinuclear ring in early prophase, the mitotic spindle and the phragmoplast indicated a pivotal role for this kinase in mitotic plant cells. So far limited research efforts have been devoted to the functions of phosphatases in the control of plant cell division. A homologue of dual phosphatase, cdc25, has not been cloned yet from alfalfa; however tyrosine phosphorylation was indicated in the case of Cdc2Ms A kinase and the p(13suc1)-bound kinase activity was increased by treatment of this complex with recombinant Drosophila Cdc25. The potential role of serine/threonine phosphatases can be concluded from inhibitor studies based on okadaic acid or endothall. Endothall elevated the kinase activity of p13(suc1)-bound fractions in G(2)-phase alfalfa cells. These biochemical data are in accordance with observed cytological abnormalities. The present overview with selected original data outlines a conclusion that emphasizes the complexity of G(2)/M regulatory events in flowering plants.

Nyckelord

abscisic acid; auxin; cell cycle phase; cytokinin; division; D-type cyclins; Medicago; mitosis; synchronization

Publicerad i

Plant Molecular Biology
2000, Volym: 43, nummer: 5-6, sidor: 595-605
Utgivare: KLUWER ACADEMIC PUBL

    UKÄ forskningsämne

    Developmental Biology
    Biochemistry and Molecular Biology

    Publikationens identifierare

    DOI: https://doi.org/10.1023/A:1006412413671

    Permanent länk till denna sida (URI)

    https://res.slu.se/id/publ/89986