Is label-free LC-MS/MS ready for biomarker discovery?

Sammanfattning

Label-free LC-MS methods are attractive for high-throughput quantitative proteomics, as the sample processing is straightforward and can be scaled to a large number of samples. Label-free methods therefore facilitate biomarker discovery in studies involving dozens of clinical samples. However, despite the increased popularity of label-free workflows, there is a hesitance in the research community to use it in clinical proteomics studies. Therefore, we here discuss pros and cons of label-free LC-MS/MS for biomarker discovery, and delineate the main prerequisites for its successful employment. Furthermore, we cite studies where label-free LC-MS/MS was successfully used to identify novel biomarkers, and foresee an increased acceptance of label-free techniques by the proteomics community in the near future.

Nyckelord

Label-free; Mass spectrometry

Publicerad i

PROTEOMICS - Clinical Applications
2015, volym: 9, nummer: 3-4, sidor: 289-294

SLU författare

• Chawade, Aakash

  • Lunds Universitet

UKÄ forskningsämne

Biokemi
Molekylärbiologi

Publikationens identifierare

• DOI: https://doi.org/10.1002/prca.201400202

Permanent länk till denna sida (URI)

https://res.slu.se/id/publ/90699