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Abstract

The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-electron microscopy in combination with methods for ab initio single-particle reconstruction and heterogeneity analysis, we have produced density maps of two conformational states of Schizosaccharomyces pombe TFIID, containing and lacking TBP. We report that TBP-binding is coupled to a massive histone-fold domain rearrangement. Moreover, docking of the TBP-TAF1(N-terminus) atomic structure to the THID map and reconstruction of a TAF-promoter DNA complex helps to account for TAF-dependent regulation of promoter-TBP and promoter-TAF interactions.

Published in

Structure
2009, volume: 17, number: 11, pages: 1442-1452
Publisher: CELL PRESS

SLU Authors

UKÄ Subject classification

Structural Biology

Publication identifier

  • DOI: https://doi.org/10.1016/j.str.2009.09.007

Permanent link to this page (URI)

https://res.slu.se/id/publ/90849